From 4ebcf363ba463ef98c2c02968117a59795e596aa Mon Sep 17 00:00:00 2001 From: Connor Laughland <71669181+conochur@users.noreply.github.com> Date: Mon, 10 Aug 2026 12:10:34 -0700 Subject: [PATCH 1/2] Update README.md added method to command line calls --- README.md | 13 +++++++------ 1 file changed, 7 insertions(+), 6 deletions(-) diff --git a/README.md b/README.md index e6cf31b..31f6413 100644 --- a/README.md +++ b/README.md @@ -10,8 +10,9 @@ We are planning on occasional updating this tool with no fixed schedule. Communi `acpreprocessing.stitching_modules.convert_to_n5.tiff_to_ngff` - Sequentially reads image arrays from a tiff stack series for pixel-wise deskew (optional), for computing a downsampling pyramid to a user-defined depth, and for writing out the data volume into a next-generation file format (zarr v3). ``` -python zarrv3_to_zarr.py \ ---input_file PATH_TO_INPUT_ZARR_FILE \ + +python -m acpreprocessing.stitching_modules.convert_to_n5.tiff_to_ngff \ +--input_dir PATH_TO_TIFF_DIR \ --output_file PATH_TO_OUTPUT_ZARR_FILE \ --group_names LIST_OF_GROUP_NAMES \ --group_attributes LIST_OF_GROUP_ATTRIBUTE_DICTS \ @@ -20,10 +21,10 @@ python zarrv3_to_zarr.py \ ``` **Point extraction** -`acpreprocessing.stitching_modules.acstitch.extract_points` - Detects blob features within user-defined ROIs of a source tile and generates initial point correspondences with a target tile using the estimated tile offset. ``` -python extract_points.py \ + +python -m acpreprocessing.stitching_modules.acstitch.extract_points \ --p_tile PATH_TO_SOURCE_TILE \ --q_tile PATH_TO_TARGET_TILE \ --output_file PATH_TO_OUTPUT_POINTMATCH_FILE \ @@ -35,10 +36,10 @@ python extract_points.py \ ``` **Tile stitching** -`acpreprocessing.stitching_modules.acstitch.stitch` - Generate point correspondences between tiles from template matching or SIFT features at user-defined resolution level (mip). ``` -python stitch_tiles.py \ + +python -m acpreprocessing.stitching_modules.acstitch.stitch \ --input_file PATH_TO_INPUT_POINTMATCH_FILE \ --output_file PATH_TO_OUTPUT_POINTMATCH_FILE \ --stitch_method STITCH_METHOD_ccorr_OR_sift \ From b4f6712c2e83274fffb4d6b2dc1d67fd5d1a370f Mon Sep 17 00:00:00 2001 From: Connor Laughland <71669181+conochur@users.noreply.github.com> Date: Mon, 10 Aug 2026 12:10:53 -0700 Subject: [PATCH 2/2] Update README.md --- README.md | 1 - 1 file changed, 1 deletion(-) diff --git a/README.md b/README.md index 31f6413..6740c14 100644 --- a/README.md +++ b/README.md @@ -7,7 +7,6 @@ We are planning on occasional updating this tool with no fixed schedule. Communi ### Methods **Deskew and zarr conversion** -`acpreprocessing.stitching_modules.convert_to_n5.tiff_to_ngff` - Sequentially reads image arrays from a tiff stack series for pixel-wise deskew (optional), for computing a downsampling pyramid to a user-defined depth, and for writing out the data volume into a next-generation file format (zarr v3). ```