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Description
Column metadata for this dataset supposedly contains experimentally determined cell cycle phase for each cell, however:
sce.ref <- scRNAseq::LengESCData()
table(sce.ref$Phase, useNA="ifany")
G1 S <NA>
91 80 289
So, I looked back at the data and the code used to create this dataset, and found that G2 in fact is present:
phase <- sub("_Exp.*", "", colnames(sce.ref))
table(phase)
phase
G1 G2 H1 S
91 76 213 80
So, this line of code:
phase[!phase %in% c("G1", "S", "G2M")] <- NA
should be changed into
phase[!phase %in% c("G1", "S", "G2")] <- NA
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