diff --git a/packages/main/cypress/specs/Menu.cy.tsx b/packages/main/cypress/specs/Menu.cy.tsx
index a5f81c24b38f..d0e0ace3e496 100644
--- a/packages/main/cypress/specs/Menu.cy.tsx
+++ b/packages/main/cypress/specs/Menu.cy.tsx
@@ -1055,8 +1055,6 @@ describe("Menu interaction", () => {
 				.should("have.attr", "accessible-name", "Select an option from the menu");
 		});
 
-		/* The test is valid, but currently it is not stable. It will be reviewed further and stabilized afterwards. */
-
 		it("Menu items - navigation in endContent", () => {
 			cy.mount(
 				<>
@@ -1071,6 +1069,10 @@ describe("Menu interaction", () => {
 				</>
 			);
 
+			// Move mouse to opener button to avoid interference with menu item hover behavior
+			cy.get("[ui5-button]")
+				.realHover();
+
 			cy.get("[ui5-menu]")
 				.ui5MenuOpen();
 
@@ -1079,24 +1081,34 @@ describe("Menu interaction", () => {
 
 			cy.get("@items")
 				.first()
-				.should("be.focused");
+				.should("be.focused")
+				.realPress("ArrowRight");
 
-			cy.realPress("ArrowRight");
-			cy.get("@buttons").first().should("be.focused");
+			cy.get("@buttons")
+				.first()
+				.should("be.focused")
+				.realPress("ArrowRight");
 
-			cy.realPress("ArrowRight");
-			cy.get("@buttons").last().should("be.focused");
+			cy.get("@buttons")
+				.last()
+				.should("be.focused")
+				.realPress("ArrowRight");
 
-			cy.realPress("ArrowRight");
-			cy.get("@buttons").last().should("be.focused");
+			cy.get("@buttons")
+				.last()
+				.should("be.focused")
+				.realPress("ArrowLeft");
 
-			cy.realPress("ArrowLeft");
-			cy.get("@buttons").first().should("be.focused");
+			cy.get("@buttons")
+				.first()
+				.should("be.focused")
+				.realPress("ArrowLeft");
 
-			cy.realPress("ArrowLeft");
-			cy.get("@buttons").first().should("be.focused");
+			cy.get("@buttons")
+				.first()
+				.should("be.focused")
+				.realPress("ArrowDown");
 
-			cy.realPress("ArrowDown");
 			cy.get("@items").last().should("be.focused");
 		});
 	});
@@ -1162,4 +1174,155 @@ describe("Menu - getFocusDomRef", () => {
 				expect(menu.getFocusDomRef()).equal(clickedItem.getFocusDomRef());
 			});
 	});
+});
+
+describe("Menu - Submenu Focus Behavior", () => {
+	it("should not move focus when submenu opens via mouse hover", () => {
+		cy.mount(
+			<>
+				<Button id="btnOpen">Open Menu</Button>
+				<Menu opener="btnOpen">
+					<MenuItem text="Parent Item">
+						<MenuItem text="Child Item 1"></MenuItem>
+						<MenuItem text="Child Item 2"></MenuItem>
+					</MenuItem>
+					<MenuItem text="Another Item"></MenuItem>
+				</Menu>
+			</>
+		);
+
+		cy.get("[ui5-menu]")
+			.ui5MenuOpen();
+
+		cy.get("[ui5-menu] > [ui5-menu-item]")
+			.as("items");
+
+		cy.get("@items")
+			.first()
+			.should("be.visible")
+			.as("parentItem");
+
+		// Hover item to open submenu
+		cy.get("@parentItem").realHover();
+
+		cy.get("@parentItem")
+			.shadow()
+			.find("[ui5-responsive-popover]")
+			.should("have.attr", "open");
+
+		// Verify focus not moved to submenu
+		cy.get("@parentItem")
+			.should("be.focused");
+
+		cy.get("[ui5-menu-item] > [ui5-menu-item]")
+			.as("submenuitems");
+
+		cy.get("@submenuitems")
+			.first()
+			.should("be.visible")
+			.as("childItem");
+
+		cy.get("@childItem")
+			.should("not.be.focused");
+	});
+
+	it("should close submenu when hover moves to another item", () => {
+		cy.mount(
+			<>
+				<Button id="btnOpen">Open Menu</Button>
+				<Menu opener="btnOpen">
+					<MenuItem text="Parent Item">
+						<MenuItem text="Child Item 1"></MenuItem>
+						<MenuItem text="Child Item 2"></MenuItem>
+					</MenuItem>
+					<MenuItem text="Another Item"></MenuItem>
+				</Menu>
+			</>
+		);
+
+		cy.get("[ui5-menu]")
+			.ui5MenuOpen();
+
+		cy.get("[ui5-menu] > [ui5-menu-item]")
+			.as("items");
+
+		cy.get("@items")
+			.first()
+			.should("be.visible")
+			.as("parentItem");
+
+		// Hover item to open submenu
+		cy.get("@parentItem").realHover();
+
+		cy.get("@parentItem")
+			.shadow()
+			.find("[ui5-responsive-popover]")
+			.as("submenuPopover");
+			
+		cy.get("@submenuPopover")
+			.should("have.attr", "open");
+
+		// Hover over another top-level item
+		cy.get("@items")
+			.last()
+			.should("be.visible")
+			.as("lastItem");
+			
+		cy.get("@lastItem")
+			.realHover();
+
+		// The original submenu should be closed
+		cy.get("@submenuPopover")
+			.should("not.have.attr", "open");
+	});
+
+	it("should move focus when submenu opens via keyboard", () => {
+		cy.mount(
+			<>
+				<Button id="btnOpen">Open Menu</Button>
+				<Menu opener="btnOpen">
+					<MenuItem text="Parent Item">
+						<MenuItem text="Child Item 1"></MenuItem>
+						<MenuItem text="Child Item 2"></MenuItem>
+					</MenuItem>
+					<MenuItem text="Another Item"></MenuItem>
+				</Menu>
+			</>
+		);
+
+		cy.get("[ui5-menu]")
+			.ui5MenuOpen();
+
+		cy.get("[ui5-menu] > [ui5-menu-item]")
+			.as("items");
+
+		cy.get("@items")
+			.first()
+			.should("be.visible")
+			.as("parentItem");
+
+		// Open submenu with keyboard
+		cy.get("@parentItem")
+			.should("be.focused")
+			.realPress("ArrowRight");
+		cy.get("@parentItem")
+			.shadow()
+			.find("[ui5-responsive-popover]")
+			.should("have.attr", "open");
+
+		// Verify focus is moved to submenu
+		cy.get("@parentItem")
+			.should("not.be.focused");
+
+		cy.get("[ui5-menu-item] > [ui5-menu-item]")
+			.as("submenuitems");
+
+		cy.get("@submenuitems")
+			.first()
+			.should("be.visible")
+			.as("childItem");
+
+		cy.get("@childItem")
+			.should("be.focused");
+	});
 });
\ No newline at end of file
diff --git a/packages/main/src/Menu.ts b/packages/main/src/Menu.ts
index 1f3e1c1f6521..a11c114dcd8e 100644
--- a/packages/main/src/Menu.ts
+++ b/packages/main/src/Menu.ts
@@ -351,7 +351,7 @@ class Menu extends UI5Element {
 		this.open = false;
 	}
 
-	_openItemSubMenu(item: MenuItem) {
+	_openItemSubMenu(item: MenuItem, openedByMouse = false) {
 		clearTimeout(this._timeout);
 
 		if (!item._popover || item._popover.open) {
@@ -365,6 +365,7 @@ class Menu extends UI5Element {
 		item._popover.opener = item;
 		item._popover.open = true;
 		item.selected = true;
+		item._openedByMouse = openedByMouse;
 	}
 
 	_itemMouseOver(e: MouseEvent) {
@@ -377,7 +378,7 @@ class Menu extends UI5Element {
 			return;
 		}
 
-		item.focus();
+		item.getFocusDomRef()?.focus();
 
 		// Opens submenu with 300ms delay
 		this._startOpenTimeout(item);
@@ -413,7 +414,7 @@ class Menu extends UI5Element {
 		this._timeout = setTimeout(() => {
 			this._closeOtherSubMenus(item);
 
-			this._openItemSubMenu(item);
+			this._openItemSubMenu(item, true);
 		}, MENU_OPEN_DELAY);
 	}
 
@@ -455,7 +456,7 @@ class Menu extends UI5Element {
 		}
 
 		if (shouldOpenMenu) {
-			this._openItemSubMenu(item);
+			this._openItemSubMenu(item, false);
 		} else if (isTabNextPrevious) {
 			this._close();
 		}
diff --git a/packages/main/src/MenuItem.ts b/packages/main/src/MenuItem.ts
index c80800c7f11b..4a11b959c3ff 100644
--- a/packages/main/src/MenuItem.ts
+++ b/packages/main/src/MenuItem.ts
@@ -319,6 +319,7 @@ class MenuItem extends ListItem implements IMenuItem {
 
 	_itemNavigation: ItemNavigation;
 	_shiftPressed: boolean = false;
+	_openedByMouse = false;
 
 	constructor() {
 		super();
@@ -535,7 +536,8 @@ class MenuItem extends ListItem implements IMenuItem {
 		if (!isInstanceOfMenuItem(item)) {
 			return;
 		}
-		item.focus();
+
+		item.getFocusDomRef()?.focus();
 
 		this._closeOtherSubMenus(item);
 	}
@@ -622,7 +624,9 @@ class MenuItem extends ListItem implements IMenuItem {
 	}
 
 	_afterPopoverOpen() {
-		this._allMenuItems[0]?.focus();
+		if (!this._openedByMouse) {
+			this._allMenuItems[0]?.focus();
+		}
 		this.fireDecoratorEvent("open");
 	}
 
@@ -644,6 +648,7 @@ class MenuItem extends ListItem implements IMenuItem {
 	}
 
 	_afterPopoverClose() {
+		this._openedByMouse = false;
 		this.fireDecoratorEvent("close");
 	}